ABSTRACT
Herbal medicines are still most popular, abundant and affordable remedies for curing various ailments. Garlina is one of the herbal formulations of Hamdard Laboratories [waqf] Pakistan used to treat cardiovascular diseases and elevated sugar level. However, there is no scientific data available regarding the potential toxicity. Therefore, the present study was to assess the acute and sub-chronic toxicity in rats. The single dose of Garlina 5000mg/kg were administered orally and observed for 14 days. A sub-chronic toxicity test was performed at 2000mg/kg of Garlina daily for 30 days. Control rats received saline. The biochemical, hematological and histopathological analysis was carried out. The acute toxicity LD50 was determined to be >5000mg/kg. The result of acute and sub-chronic toxicity revealed no mortality and sign of toxicity. Garlina did not elicit any significant change in body weight, hematological and histopathology analysis when compared to saline treated rats. The relative weight of organs was not affected by the treatment. While the daily dose of Garlina for humans is 20mg/kg. However, the sub-chronic toxicity at 2000mg/kg dose of Garlina exhibited significant increase in gamma glutamyltransferase while total protein significantly decreased. Results obtained from study demonstrated that there is wide margin of safety for the therapeutic use of Garlina and significant decrease in LDL, atherogenic index, GGT and bilirubin direct at the dose of 5000mg/kg further strengthen the use as hypolipidemic and hypoglycemic agent
ABSTRACT
Primary hyperlipidemia and atherosclerosis are correlated to each other and these are major cibdutuuibs of coronary artery diseases. The objective of the study was to evaluate the efficacy of polyherbal Unani medicine [Garlina] a well-known herbal formulation to be used for Primary hyperlipidemia. This was an open, randomized, prospective, multicenter study on human subjects of 6 months duration. After the laboratory investigations for fasting plasma lipids of the suspected subjects, 25 subjects were identified, randomized and received the drug. The subjects were diagnosed and classified according to the international recommended levels of plasma lipids [PL] as total cholesterol levels were borderline high [200-239 mg/dL], triglycerides [TGs] were high [200-499 mg/dL], high density lipoprotein [HDL] were desirable but not optimal [41-59 mg/dL] while low density lipoproteins [LDL] were borderline high [130-159 mg/dL]. After 8 weeks administration of Garlina tablets 500 mg twice-a-day, the total cholesterol levels were reduced to 166.48+/- 35.59 mg/dL from 224.04+/-9.16 mg/dL, triglyceride levels were also reduced to 144.28+/- 37.11 mg/dL from 217.28+/-73.54 mg/dL at the base line, low density lipoprotein levels were reduced to S7.52+/-40.17 mg/dL from 133.96+/- 54.00 mg/dL while high density lipoprotein level was increased to 56.20±8.66 mg/dL from 48.28+/-10.57 mg/dL. The findings from this study demonstrated that there was significant difference [p<0.05] before and after treatment with Garlina [500 rqg] as mean lipid profiles of test group administered 1 tablet b.d. after meals. This is clearly evident that Garlina possesses a significant hypolipidemic therapeutic value for the treatment of primary hyperlipidemia
ABSTRACT
The purpose of this study was to determine the safety profile of a polyherbal drug, Garlina. The acute toxicity was carried out at 5000 mg/kg administered orally while sub-chronic toxicity was assessed by daily oral dosing of 2000mg/kg in rabbits following ABPI and BTS guideline, 2009 and EMA, 2000, respectively. The outcomes of Garlina-treated group were compared with control group that received saline. The results of Garlina administered orally once at a dose of 5000 mg/kg and 2000 mg/kg/ day consecutively for a period of one month does not produce any remarkable adverse effects when analyzed histopathologically or biochemically. Similarly, hematological profile, body and organs weight also remained unchanged in its presence. However, a significant increase in uric acid at a single dose of 5000 mg/kg and a decline in RBCs count at the dose of 2000 mg/kg of Garlina were observed that will be monitored cautiously in the on-going clinical trials. The findings obtained in this study suggest that at prescribed dose Garlina is relatively safe for human consumption
ABSTRACT
The Purpose of this research study was to examine the lipid lowering activity of a traditionally used poly herbal product "Mufarreh Yaqooti Motadil [MUYM], in Egg yolk induced hyperlipidaemic rats. The product is being manufactured by Hamdard Laboratories [waqf] Pakistan for the last thirty years and has shown significant therapeutic effects. During our study the product was found to have protective effects against hyperlipidemia in Human dose. The activity was performed by the help of Biochemical Investigation, using Spectrophotometric method. The results were justified by the presence of scientific data during the retrospective literature search of the Product's Ingredients. A comparative study with the Allopathic Medicine used for Antihyperlipidemic activity was also performed. Significant effect of Herbal Drug on LDL and HDL and Triglyceride levels were observed which was statistically comparable with that of Atorvastatin
Subject(s)
Animals, Laboratory , Phytotherapy , Egg Yolk , Hypolipidemic Agents , Rats, Sprague-Dawley , Heptanoic Acids/pharmacology , Heptanoic Acids , Anticholesteremic AgentsABSTRACT
Many plants have been recommended in alternative systems of medicine for hypotensive activity although few systematic studies have been done. In this study, the hypotensive activity was studied in normotensive albino rats at different doses of BRPM and BB. The receptor activity was assessed by the drugs Ach on rat heart. Administration of different methanolic extract [BRPM, BB] showed significant reductions in blood pressure comparable to its respective control. Both methanolic extract completely blocked the Ach receptors in heart. This study suggests that the methanolic extract [BRPM, BB] have significant Ach receptor blocking and hypotensive activity and may be recommended for further investigation
Subject(s)
Animals, Laboratory , Phytotherapy , Antihypertensive Agents , Rats, Sprague-Dawley , Plant ExtractsABSTRACT
Mufarreh Yaqooti Motadil was investigated for its toxicological activity in human dose, in rats. The drugs was found to be non-toxic and well tolerated even if treated for a long period of time. The biochemical studies revealed that drug decreased the serum level of cholesterol, triglycerides, glucoseand bilirubin non-significantly [P>0.05]. On liver the drug showed very good affects as caused a significant decrease [P<0.05] in GGT, SGPT and SGOT
ABSTRACT
The present study was conducted to investigate the physiological and pharmacological effect of Somina [herbal Preparation] on cardiac activity of Frog. The dose of Somina [20mg/ml] competitively acts on muscarinic receptor and reduced the heart rate while increased the cardiac force and duration of cardiac cycle. It is therefore concluded that somina drug regulates the cardiac activity by increasing efficiency of heart
ABSTRACT
The effects of Somina at the dose of 285-mg/kg were studied on the dopamine level in rat brain. Somina resulted in a significant increase in dopamine content. The increased concentrations of the neurotransmitters Dopamine in rat brain might be responsible to improve memory processes and reduces neuropsychiatric disorder